Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: Consensus motives for the GDP-GTP binding site are boxed in black. Mutated and amino acids of interest are boxed in grey. The sequence of the C-terminal peptide synthesised for the production of the rabbit immune serum is underlined. Databanks accession numbers: Ld ARL-1, Genbank AF187855 and AAF29899; La ARL-1, GenBank XXX; Lm ARL-1, GeneDB LmjF17.0070 ( http://www.genedb.org/ ) ; Tb ARL-1, Genbank AAX70381, GeneDB Tb927.7.6230, previously Tb07.2F2.550; Hs ARL-1, Swissprot P40616; ScARL-1: Genbank S46035; Hs ARF-1: Swissprot P32889; Hs ARF-6: Genbank NP_001654.

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Binding Assay, Sequencing

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: Extracts of 3.10 6 exponentially growing L. amazonensis promastigotes (about 3 µg of proteins) were submitted to western blot analysis using the rabbit anti- Ld ARL-1 C-terminus immune serum (1∶5000 dilution); color was developed with an anti-rabbit IgG peroxidase conjugate (1∶10000 dilution) and an ECL revelation kit (Amersham). Internal standard: 37 kDa LACK antigen. Left, negative control without anti-LdARL-1 antiserum. Additional bands are revealed when the GFP-fused proteins are expressed, probably due to partial degradation when the cells were lysed, in spite of the presence of antiproteases (see ).

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Western Blot, Negative Control

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: A. IF on fixed cells: A1, phase contrast; A2, co-labelling with DAPI (blue) and FITC-anti rabbit-IgG plus anti- Ld ARL-1 rabbit immune serum (green). B. IF on fixed cells co-transformed with pNUS- Ld ARL-1-GFPcH (green) and pNUS-mREDnD- Ld LPG-2 (red) plus DAPI staining (blue). C. IF on fixed cells co-transformed with pNUS- Ld ARL-1-GFPcH (C1, green) plus pNUS- Ld ARF-1-mRedcD (C2, red), overlay (C3). D. EM of cells transformed with pNUS- Ld ARL-1-GFP and incubated with anti- Ld ARL-1 immune serum. C1 and C2 are sections of two different cells. Black dots (10 nm gold particles) show the Ld ARL-1 immunoreactive spots. N: nucleus; G: Golgi apparatus; K: kinetoplast; F: flagellum; FP: flagellar pocket.

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Transformation Assay, Staining, Incubation

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: Fixed L. amazonensis promastigotes transformed with: A, pNUS- Ld ARL-1-GFPcH (green); B, pNUS- Ld ARL-1/G2A-GFPcH (green).

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Transformation Assay

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: Fixed L. amazonensis promastigotes expressing in red Ld ARF-1-mRed (5B, 5E, 5H) and in green Ld ARL-1/Q74L-GFP (GTP) (5A), Ld ARL-1/T51N-GFP (GDP) (5D), Ld ARL-1/T34N-GFP (empty) (5H); overlays are in 5C, 5F and 5I. fp, flagellar pocket.

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: Fixed L. amazonensis promastigotes with plasma membrane labelled in red with Concanavalin A-Texas Red. A, cell expressing in green Ld ARL-1-GFP. B-C, cell expressing in green Ld ARL-1/T51N-GFP (GDP); D-F, cell expressing in green Ld ARL-1/T34N-GFP (empty). The yellow signal is an overlay of green and red signals.

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: L. amazonensis promastigotes expressing Ld ARL-1-GFP (A–D, green) or Ld ARL-1/T34N-GFP (empty) (E–H, green) were incubated at 24°C for 15 min with 2 µg/ml FM4-64FX (red), washed and further incubated for 0 min (A, E), 15 min (B, F), 60 min (C, G) and 120 min (D, H) before fixation; fp, flagellar pocket; MVT, Lysosome/Multivesicular Tubule. Colocalization of red and green results in a yellow signal.

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Expressing, Incubation

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: BA125 L. amazonensis promastigotes expressing in red the mRed-DPMS and in green Ld ARL-1-GFP (A), Ld ARL-1/G2A-GFP (unMyr) (B), Ld ARL-1/Q74L-GFP (GTP) (C), Ld ARL-1/T51N-GFP (GDP) (D) or Ld ARL-1/T34N-GFP (empty) (E).

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: A. Fixed L. amazonensis promastigotes expressing in green Ld ARL-1-GFP (1), Ld ARL-1/Q74L-GFP (GTP) (2), Ld ARL-1/T51N-GFP (GDP) (3), Ld ARL-1/G2A-GFP (unMyr) (4) or Ld ARL-1/T34N-GFP (empty) (5) and stained in red with the rabbit anti- Tb VP1 (acidocalcisomes marker) immune serum plus anti-rabbit IgG-Texas-Red conjugate. B. Live L. amazonensis promastigotes expressing in green Ld ARL-1-GFP (1-2), Ld ARL-1/T34N-GFP (empty) (3-4) or Ld ARL-1/T51N-GFP (GDP) (5-6) and stained with DAPI: in Blue, nucleus and kinetoplast, in yellow: polyphosphates of acidocalcisomes .

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Expressing, Staining, Marker

Journal: PLoS ONE

Article Title: The Leishmania ARL-1 and Golgi Traffic

doi: 10.1371/journal.pone.0001620

Figure Lengend Snippet: Overlay of fixed L. amazonensis promastigotes expressing in red mRed- Ld pGRIP-2 and in green Ld ARL-1-GFP (A), Ld ARL-1/Q74L-GFP (GTP) (B), Ld ARL-1/G2A-GFP (unMyr) (C), Ld ARL-1/T51N-GFP (GDP) (D) and Ld ARL-1/T34N-GFP (empty) (E). Overlay of red and green is yellow.

Article Snippet: An anti- Ld ARL-1 C-terminus rabbit immune serum was prepared as described for Ld ARL-3A using an Ld ARL-1 C-terminal synthetic peptide (NH 2 -MDWLVERLREQGIGA-COOH, Genosphere Biotech) and a «Fauve de Bourgogne» rabbit (Centre d'Elevage Janvier).

Techniques: Expressing

Journal: Cell reports

Article Title: ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly

doi: 10.1016/j.celrep.2021.110139

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies against NDUFB8, SDHA, UQCRC2,COXI, ATPase-a, vinculin and VDAC1 were obtained from Abcam (1:1000); β-actin and tubulin were obtained from Millipore Sigma (1:5000); Rabbit anti-ATAD3 was obtained from GenScript pre-immune Serum 1 (SC1088) (1:500); TUJ1 was obtained from Covance (1:10,0000); and Anti GAPDH from Ambion (1:10,000).

Techniques: Virus, Mutagenesis, RNA Sequencing, Knock-Out, Recombinant, Plasmid Preparation, Software